Laboratory Pulsed Field Gel Electrophoresis Factory - Intelligent Thermal cycler – NANBEI

Instrument Construction

Figure 1. Frontal view of the thermal cycler.

● Reaction module bay — holds inserted reaction module
● Air vents — allows the thermal cycler to cool quickly
● Status LED — indicates status of reaction module
● LCD display — displays operating status
● USB A port — connects to a USB key, computer mouse, or other USB devices

Figure 2. Back view of the thermal cycler.

● Connector — connection between host machine and reaction module
● Reaction module locking screw —locks reaction module
● Test port — for service testing only
● Ethernet port — connects the thermal cycler to a computer

Figure 3. The lid and cooling fins of a 96-well reaction module.

● Lid pressure adjustment wheel — adjust the lid pressure
● Unlock pin — to unlock wheel
● Lid pull — opens and closes the lid
● Connector — connection between host machine and reaction module

Figure 4. Opening view of a 96-well reaction module.

●Inner lid — maintains the lid temperature to prevent condensation and evaporation
● Reaction block — holds reaction vessels, including tubes and microplates

C. High Performance Smart Lid
To achieve optimum pressure on the tubes the GE9612T-S is equipped with a height adjustable heated lid.
Close the lid:
After the samples have been placed in the block close the lid. Turn the wheel clockwise until you hear a clicking noise. In this mode the pressure will not increase further, even when you keep on turning the wheel.
Note: The pressure of the lid has been optimised for a fully loaded block. If only very few
Tubes are loaded to the block you should place dummy tubes in the four corner positions to avoid damage of tubes by excessive pressure.
Open the lid:
First: Release pressure by turning the wheel counter clockwise. As soon as there is no more
resistance the pressure has been released.
Then: Open the lid with by pushing the front button.
Important: The lid should not be opened under pressure because this leads to damage of the locking mechanism.

D. Releasing blocked lid wheel
Note: When the lid is in the very up or down position, it may happen that the wheel is
uncoupled. In this situation the clutch mechanism is active in both directions (clicking noise in
either direction).
To unlock wheel, press down metal pin with a ball pen and turn wheel carefully. This pin
overrides the automatic clutch mechanism. Thus, care must be taken not to apply excessive
pressure.

Release lid in upper position:
1)press pin
2)carefully turn wheel while holding the pin down CLOCKWISE, until you feel normal resistance (no more clicking noise, clutch is released). Release pin and turn lid down, until
the clutch mechanism is activated (clicking noise, optimum pressure applied).
Release lid in down position:
1)press pin
2)carefully turn wheel while holding the pin down COUNTERCLOCKWISE, until you feel
normal resistance (no more clicking noise, clutch is released). Release pin and turn wheel counter clockwise until pressure is completely released.Open lid.
Important: When the clutch mechanism is active (= optimum pressure is applied), do not use pin to further increase lid pressure. This would lead to damage of tubes and instrument !

Specialty

Two blocks independently controlled and can run 2 different PCR programs simultaneously;
Stepless adjustable hot lid with pressure-protection, fit tubes of different heights to avoid tube melt and evaporation;
Windows interface, 8” ( 800×600, 16 colors) TFT color touch-screen with graphical display provides easy use for setting up and monitoring;
Built-in 11 standard program file template, can quickly edit the required files;
Folder management, user can build directory;
The running program and left time can be displayed in real time , allow to edit file when program is running;
One-click quick incubation function can meet experiment’s needs such as denaturation, enzyme cutting/enzyme-link and ELISA;
Internal flash memory for 10000 typical PCR files in free configurable folders;
Hot lid temperature and hot lid work mode can be set to meet different experiment’s need;
Automatic restart after power failure. When power is restored it can continue to run unfinished program;
GLP report records every step to provide accurate data support for experiment result analysis;
User Login Management, three-tier permission, password protection function to ensure data security;
Compatible with devices such as Mouse and Keyboard and capable to transfer data and perform software updates via USB Drive;
Support USB and LAN to update software;
One computer can control many sets of PCR via network connection;
Support email-alert function when experiment is over.

Performance